RHIZOBAKTERIA PENGHASIL FITOHORMON IAA PADA RHIZOSFIR TUMBUHAN SEMAK KARAMUNTING, TITONIA, DAN TANAMAN PANGAN

Agustian Agustian, Nuriyani Nuriyani, Lusi Maira, Oktanis Emalinda

Abstract


Rhizobacteria from various research results have shown an important role in producing organic compounds (phytohormone) which can affect plant physiological processes even in low concentrations. This research aimed to study the production of Indole Acetic Acid (IAA) in several plants rhizosphere, to calculate the rhizobacteria population that able to produce and synthesize IAA and to isolate these rhizobacteria from several rhizospheres of cultivated plants (maize and peanut) and bush plant i.e. karamunting (Rhodomyrtus tomentosa) and titonia (Tithonia diversifolia). All plants and crops were obtained from experimental station Faculty of Agriculture Andalas University. Further research is conducted in Soil Biology Laboratory Faculty of Agriculture. Experimental design in this study was not used but the data obtained from 3 replications tested by T test at 5% level and if it was significantly different it would be followed by further HSD tests at 5% level. Highest content of IAA was found in the peanut crop (67.30 ppm), followed by maize (53.61 ppm) and 28.53 ppm for titonia. Whereas the lowest content of IAA was found in karamunting rhizosphere, it was 22.29 ppm. The highest amount of rhizobacteria was obtained from peanut rhizosphere followed by maize and titonia i.e 18.28, 12.08 and 7.87% of the total population, respectively. There was no IAA producing rhizobacteria population was found in karamunting rhizosphere. Based on the results of the ability of the test to produce IAA in the King’s B liquid medium at low pH (pH 4.0), the leading isolates were obtained in each rhizosphere, namely: J.2b and J.3b isolates from maize rhizosphere; Kc.1b, Kc.2b and Kc.3b from peanut isolates, and Ti.3c isolates from titonia rhizosphere.

Key words : Rhizobacteria, IAA, Rhodomyrtus tomentosa, Tithonia diversifolia, peanut and maize


Full Text:

PDF

References


Akbari, A., S.M. Arab, H.A Alikhani, I. Allahdadi dan M.H. Arzanesh. 2007. Isolation and Selection of Indigenous Azospirillum spp and the IAA of Superior Strains Effect on Wheat Roots. World Journal of Agricultural Sciences 3(4): 523-529

Balai Penelitian Tanah. 2004. Prosedur pengambilan contoh tanah untuk analisis mikroba. Pusat Penelitian dan Pengembangan Tanah dan Agroklimat.

Barea, J. M., E. Navarro, and E. Montoya. 1976. Production of plant growth regulators by rhizosphere phosphate-solubilizing bacteria. J. Appl. Bacteriol. 40: 129-134

Barea, J.M., M.J. Pozo, R. Azcón and C. Azcón-Aguilar. 2005. Microbial co-operation in the rhizosphere. J.of Exp. Bot., Vol. 56, No. 417, pp. 1761–1778

Brick, J.M., R.M. Bostock, S.E Silvertone. 1991. Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Env. Microbiol. 57: 535-538

Broeckling, C.D., A.K Broz, J. Bergelson, D.K. Manter, and J. M. Vivanco. 2008. Root Exudates Regulate Soil Fungal Community Composition and Diversity. Appl. and Env. Microbiol, p. 738–744

Camargo-Ricalde, S.L., and S.S. Dhillon. 2003, Endemic mimosa species can serve as mycorrhizal “resource islands†within semiarid communities of the Tehuacan-Cuicatlan Valley, Mexico. Mycorrhiza. 13(3): 129-136

Cattelan, A.J., P.G. Hartel, and J.J. Fuhrmann. 1999, Screening for plant growth-promoting rhizobacteria to promote early soybean growth. SSSAJ 63;

Curl, E dan T. Bryan. 1985. The Rhizosphere. Springer-Verlag. Berlin Heidelberg New York. Tokyo. 290 halaman

Dommergues, Y. and F. Mangenot. 1970. Ecologie Microbienne du Sol. Paris Masson et Cie. pp 796

Fitter, A.H dan R.K.M. Hay. 1991. Fisiologi lingkungan tanaman (terjemahan Sri Andani dan E.D. Purbayanti “Plants fisiologyâ€). Gadjah Mada University Press. Yogyakarta. 421 halaman

Foth, H. D. 1998. Dasar-dasar ilmu tanah (terjemahan Purbayanti, Lukiwati dan Trimutsih â€Fundamentals of soil Scienceâ€). Gadjah Mada University Press. Yogyakarta. 782 halaman

Frankenberger, Jr.,W.T., and M. Arshad. 1995. Phytohormones in soils. Microbial production and function. Marcel Dekker, Inc. New York. pp. 503

Fuhrmann, J. J. 1998. Microbial metabolisms. In Principles and Applications of Soil Microbiology (Editors: Sylvia D.M., JJ Fuhrmann, P.G. Hartel, D.A Zuberer) Prentice Hall, New Jersey, pp 189-217

Glick, B.R. 1995. The enhancement of plant growth by free-living bacteria. Can J. Microbiol. 41: 109-117

Hakim, N dan Agustian. 2003. Gulma Titonia dan Pemanfaatannya Sebagai Unsur Hara untuk Tanaman Hortikultura. Laporan Penelitian Hibah Bersaing XI/I Perguruan Tinggi. Fakultas Pertanian Universitas Andalas. 62 hal.

__________________________,. Oksana. E.Fitra., and R. Zamora,. 2004. Amelioration of acid soil infertility by (Titonia diversifolia) green manure and lime application. In Proceeding 6th International Symposium Plant-Soil Interaction at low pH (PSILPH) on 1-5 August 2004 in Sendai Japan. pp 366-367

________________________. 2005a. Cultivation of (Titonia diversifolia) as a sources of organic matter and plant nutrients. In Proceeding 15th International Plant Nutrition Colloquium on 14-19 September, 2005. C.J.Li et al (eds). Plant Nutrion for Food Security, Human Health and Environmental Protection.. Tsinghua University Press. Beijing-China. pp 996-997

_________________________. 2005b. Budidaya titonia dan pemanfaatannya dalam usaha tani tanaman hortikultura dan tanaman pangan secara berkelanjutan pada Ultisol. Laporan Penelitian Tahun III Hibah Bersaing XI/III. Proyek Peningkatan Penelitian Perguruan Tinggi DP3M Ditjen Dikti. Lembaga Penelitian Unand. Padang

Hanafiah, K.A dan I. Anas, A. Napoleon, N. Ghoffar. 2005. Biologi tanah (ekologi dan makrobiologi tanah ). Grafindo Persada. Jakarta. 165 halaman

Haryati. 1987. Pengaruh zat pengatur tumbuh terhadap pertumbuhan stek tanaman anggur. Makalah Fakultas Pertanian Universitas Andalas. Padang. 21 halaman

Imas, T., R. S. Hadioetomo, A. W. Gunawan, dan Y. Setiadi. 1989. Mikrobiologi tanah II. Institut Pertanian Bogor. Bogor.

Jama, B.A., C.A. Palm, R.J. Buresh, A.I. Niang, C. Gachengo, G. Nziguheba, and B. Amadalo. 2000. Titonia diversifolia as a green manure for soil fertility improvement in western Kenya : a review. Agroforestry Systems. 49; 201-221

Klement, Z,K., Rudolph, K dan Sand D.C. 1990. Methods In Phytobacteriology. Budapest. Academic Kiado. 57 hal.

Kloepper, J. W. 1993. Plant growth-promoting rhizobacteria as biological control agents. In Soil microbial ecology. (Editor: Metting, F. B. Jr.). Marcel Dekker, Inc. New York. pp. 255-274.

Kloepper, J.W., M.S.Reddy, D.S. Kenney, C. Vavrina, N. Kokalis-Burelle, and N. Martinez-Ochoa. 2004. Applications for rhizobacteria in transplant production and yield enhancement. Acta Hort. 631: 219-229

Lauriks, R., R. De Wulf, S.E. Carter and A.I. Niang. 1999. A methodology for the description of boder hedges and the analysis of variables influencing their distribution: a case study in Western Kenya. Agroforestry Systems 44: 69-86

Lebuhn,M., T. Heulin, and A. Hartmann. 1997. Production of auxin and other indolic and phenolic compounds by Phaenibacillus polymixa strains isolated from different proximity to plants root. FEMS Microbiol. Ecol. 22:325-334

Lee, M., C. Breckenridge, R. Knowless. 1970. Effect of some culture conditions on the production of indole acetic acid and gibberellin like substances by Azotobacter vinelandii. Can. J. Microbiol.16: 1325-1330

Lingga, P. 1986. Petunjuk penggunaan Kapur. PT. Penebar Swadaya. Aggota IKAPI. Jakarta. 48 halaman

Narula, N., A. Deubel, W. Gans, R.K. Behl, W. Merbach. Paranodules and colonization of wheat roots by phytohormone producing bacteria in soil. Plant Soil Environ. 52, 2006 (3): 119–129

Paul, E.A., and F.E Clark. 1996. Soil Microbiology and Biochemistry. Academic Press. New York. pp 340

Rutunga, V., N.K. Karanja, C.K.K. Gachene; and C.A. Palm.1999. Biomass production and nutrient accumulation by Tephrosia vogelli and Titonia diversifolia fallows during six month growth at Maseno. Biotechnology, Agronomy, Soc. and Environment.3: 237-246.

Salisbury, F. B dan C.W. Ross. 1985. Fisiologi Tumbuhan. Jilid 2 (terjemahan). Institut Teknologi Bandung. Bandung. 173 halaman

Sanchez, P.A. and B.A. Jama. 2000. Soil fertility replenishment takes off in East and Southern Africa. Intenational Symposium on Balanced Nutrient Management Systems for the Moist Savanna and Humid Forest zones of Africa.Held on 9 Oct 2000 in Benin, Africa. 32 pp

Sarief, S. 1986. Kesuburan dan pemupukan tanah, Pustaka Buana. Bandung. 182 halaman

Sarwar, M., M. Arshad, D.A Martens, dan W.T. Frankenberger Jr.1992. Tryptophan-dependent biosynthesis of auxins in soil. Plant and Soil 147: 207-215

Schlegel, H.G. dan Schmidt, K. 1984. Mikrobiologi Umum (Edisi 4, terjemahan). Gadjah Mada University Press. Yogyakarta. 686 halaman

Sutedjo, M.M dan A.G. Kartasapoetra, RD.S. Sastroatmodjo. 1991. Mikrobiologi tanah. Rineka Cipta. Jakarta. 447 halaman

Thakuria, D., N.C. Talukdar, C. Goswami, S. Hazarika, R.C. Boro and M.R. Khan. 2004. Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Current Science, VOL. 86. p. 978-985

Weaver. J.W. 1972. Plant growth substance in agriculture. W.H Freeman and Co. San Fransisco. 418 halaman

Wollum, A.G. 1994. Soil sampling for microbiological analysis in Methods of Soil Analysis Part 2 Microbiological and Biochemical Properties ed R.W. Weaver et.al. Soil Sci. Soc. Am. Inc

Wollum, A.G. 1988. Zat pengatur tumbuh tanaman. Pusat Antar Universitas Bioteknologi IPB. Bogor. 145 halaman

Weaver. J.W. 1972. Plant growth substance in agriculture. W.H Freeman and Co. San Fransisco. 418 halaman

Wedastri, S. 2002. Isolasi dan seleksi Azotobacter spp. penghasil faktor tumbuh dan penambat nitrogen dari tanah masam. Jurnal Ilmu Tanah dan Lingkungan vol. 3. halaman 45 – 51.




DOI: http://dx.doi.org/10.25077/js.7.1.49-60.2010

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

ISSN: 2356-0835